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igg2b antibody isotype control mpc 11  (Bio X Cell)


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    Bio X Cell igg2b antibody isotype control mpc 11
    Igg2b Antibody Isotype Control Mpc 11, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 94/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+igg2b+isotype+control+mpc-11+antibody/InVivoPlus+mouse+IgG2b+isotype+control%2C+unknown+specificity/pmc07174077-38-15-23
    Average 94 stars, based on 67 article reviews
    igg2b antibody isotype control mpc 11 - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Control:

    Article Title: Transcriptomic profiling of mouse mammary tumors enables prognostic and predictive biomarker discovery for human breast cancers
    Article Snippet: .. The following antibodies and doses were used: anti-PD1 (250 ug, Bio X Cell, BE0146, RRID:AB_10949053), anti-CTLA4 (125 ug, Bio X Cell, BE0164, RRID:AB_10949609), CD40AG (100 ug, Bio X Cell, BE0016-2, RRID:AB_1107647), IgG2a isotype control (125 ug, Bio X Cell, BE0089, RRID:AB_1107769), and IgG2b isotype control (125 ug, Bio X Cell, BE0086, RRID:AB_1107791). ..

    Article Title: A ligand-mimetic anti-TREM2 agonist antibody elevates soluble TREM2 and ameliorates pathology in mouse models of Alzheimer's disease and multiple sclerosis.
    Article Snippet: 265 ACCEPTED MANUSCRIPT AR TIC LE IN PR ES S ARTICLE IN PRESS Page 13 266 In vitro phagocytosis assay 267 6 x 104 cells/well of mouse microglial cell line BV-2 (AcceGen Biotechnology, 268 #ABC-TC212S) were plated in 96-well plates. .. The next day, cells were treated with or 269 without 30 nM anti-TREM2 antibody 03O05 or mouse IgG2b isotype control (msIgG2b, 270 bioxcell, #BP0086) for 2 to 24 hours, and 10 μM cytochalasin D (Invitrogen, #PHZ1063) 271 was included as a negative control. .. At the end of the treatment, the media was replaced 272 with Live Cell Imaging Solution (Invitrogen, #A59688DJ) containing 0.5 mg/ml pHrodo 273 Green E.coli BioParticles Conjugate for Phagocytosis (Invitrogen, #P35366), and cells 274 were incubated at 37°C without elevated CO2 for 3 hours.

    Article Title: IL-35 produced by dendritic cells via TIM-3-STAT3 signaling contributes to the development of visceral leishmaniasis
    Article Snippet: The following antibodies were used for flow cytometry: PE-anti-mouse EBI3 (IC18341P) and APC-anti-human/mouse IL-12p35 (IC2191A) (both from R&D Systems); eFluor 660-anti-mouse IL-12p35 (50-7352-82), PerCP-anti-mouse/human IL-12p35 (MA5-23622) and Alexa Fluor 594-anti-mouse IgG (A-11020; all from Thermo Fisher Scientific); PE-anti-human EBI3 (360903), FITC-anti-mouse CD40 (124608), FITC-anti-mouse CD86 (105006), FITC-anti-mouse CD80 (104706), FITC-anti-mouse CD11c (117306), PE-anti-mouse CD8α (100708), FITC-anti-mouse CD8α (100706), PE/Cyanine7-anti-mouse CD3 (100220), PerCP/Cyanine5.5-anti-mouse CD4 (100434), Alexa Fluor 647-anti-mouse IDO1 (654003), APC-anti-mouse IL-10 (505010), FITC-anti-mouse IFNγ (505806) and isotype control antibodies such as FITC-rat IgG2a,κ (400505) and FITC-armenian hamster IgG (400905; all from Biolegend, CA, USA). .. Neutralization studies were performed using neutralizing anti-IL-35 (clone V1.4C4.22, MABF848; Sigma-Aldrich) and the corresponding isotype control mouse IgG2b (BE0086; Bio X Cell, NH, USA). .. The TIM-3 blocking antibody (clone RMT3-23, BE0115) ( Chiba et al ., 2012 ) and the isotype control rat IgG2a,κ (BE0089) were obtained from Bio X Cell.

    Article Title: Removal of Interstitial Hyaluronan Facilitates Subcutaneous Administration and Lymphatic Delivery of Anti-CTLA4 and Improves Antitumor Efficacy
    Article Snippet: Subcutaneous administration is an increasingly patientpreferred, alternative route of administration for monoclonal antibodies (mAb).. To overcome the dose-volume restriction with subcutaneous administration and enable the large mAb doses typically required for immunotherapy, recombinant human hyaluronidase PH20 is co-dosed to transiently depolymerize hyaluronan at the injection site.. Despite increasing clinical approvals and clinical trials of combination products with PH20, the potential impact of PH20 in facilitating intravenous-to-subcutaneous dose switching is largely unknown.

    Article Title: IL-35 enhances IL-10⁺ breg-mediated immunoregulation and attenuates inflammation and fibrosis in systemic sclerosis
    Article Snippet: .. Additionally, both the mAbIL-35 group and its corresponding isotype control antibody (InVivoPlus mouse IgG2b isotype control, BioXcell, USA, Cat# BP0086) group were included in the BLM mouse model, with results provided in Supplementary Material 1. ..

    Negative Control:

    Article Title: A ligand-mimetic anti-TREM2 agonist antibody elevates soluble TREM2 and ameliorates pathology in mouse models of Alzheimer's disease and multiple sclerosis.
    Article Snippet: 265 ACCEPTED MANUSCRIPT AR TIC LE IN PR ES S ARTICLE IN PRESS Page 13 266 In vitro phagocytosis assay 267 6 x 104 cells/well of mouse microglial cell line BV-2 (AcceGen Biotechnology, 268 #ABC-TC212S) were plated in 96-well plates. .. The next day, cells were treated with or 269 without 30 nM anti-TREM2 antibody 03O05 or mouse IgG2b isotype control (msIgG2b, 270 bioxcell, #BP0086) for 2 to 24 hours, and 10 μM cytochalasin D (Invitrogen, #PHZ1063) 271 was included as a negative control. .. At the end of the treatment, the media was replaced 272 with Live Cell Imaging Solution (Invitrogen, #A59688DJ) containing 0.5 mg/ml pHrodo 273 Green E.coli BioParticles Conjugate for Phagocytosis (Invitrogen, #P35366), and cells 274 were incubated at 37°C without elevated CO2 for 3 hours.

    other:

    Article Title: A Developmental Lectin-Glycan Program Enables Early Breast Cancer Dissemination and Metastatic Onset
    Article Snippet: Secondary antibodies used were Alexa Fluor 488 goat anti-mouse (Abcam ab150113; 1:1000) and Alexa Fluor 568 goat anti-rabbit (Abcam ab150080; 1:1000).

    Neutralization:

    Article Title: IL-35 produced by dendritic cells via TIM-3-STAT3 signaling contributes to the development of visceral leishmaniasis
    Article Snippet: The following antibodies were used for flow cytometry: PE-anti-mouse EBI3 (IC18341P) and APC-anti-human/mouse IL-12p35 (IC2191A) (both from R&D Systems); eFluor 660-anti-mouse IL-12p35 (50-7352-82), PerCP-anti-mouse/human IL-12p35 (MA5-23622) and Alexa Fluor 594-anti-mouse IgG (A-11020; all from Thermo Fisher Scientific); PE-anti-human EBI3 (360903), FITC-anti-mouse CD40 (124608), FITC-anti-mouse CD86 (105006), FITC-anti-mouse CD80 (104706), FITC-anti-mouse CD11c (117306), PE-anti-mouse CD8α (100708), FITC-anti-mouse CD8α (100706), PE/Cyanine7-anti-mouse CD3 (100220), PerCP/Cyanine5.5-anti-mouse CD4 (100434), Alexa Fluor 647-anti-mouse IDO1 (654003), APC-anti-mouse IL-10 (505010), FITC-anti-mouse IFNγ (505806) and isotype control antibodies such as FITC-rat IgG2a,κ (400505) and FITC-armenian hamster IgG (400905; all from Biolegend, CA, USA). .. Neutralization studies were performed using neutralizing anti-IL-35 (clone V1.4C4.22, MABF848; Sigma-Aldrich) and the corresponding isotype control mouse IgG2b (BE0086; Bio X Cell, NH, USA). .. The TIM-3 blocking antibody (clone RMT3-23, BE0115) ( Chiba et al ., 2012 ) and the isotype control rat IgG2a,κ (BE0089) were obtained from Bio X Cell.



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